The abundance and distribution of arbuscular mycorrhizal fungi (AMF) in forest soils depends upon environmental conditions and habitat disturbance. We investigated the effect of three plant communities and soil properties on the AMF spore density and root colonization and soil properties in a dry Afromontane forest. Soil and root samples were collected from 65 permanent plots of 50 m x 50 m, assessed for AMF spore density and root colonization from four sides of each tree plant, and replicated three times for dominant woody species across two soil depths. Analysis of soil chemical properties and determination of soil carbon stock were conducted across two soil depths. All three-plant communities were colonized by AMF. Juniperus procera-Maytenus senegalensis community (Jupr-Mase) and Cadia purpurea-Opuntia ficus indica community (Capu-Opfi) had the highest and lowest AMF spore density and root colonization respectively. Pterolobium stellatum-Celtis africana community (Ptst-Ceaf) did not show significant difference with Jupr-Mase and Capu-Opfi. The top soil (0-25 cm) had significantly (p < 0.05) higher AMF spore density but lower root colonization percentage than the sub-soil (25-50cm). We also found significant correlation (p < 0.05) between soil carbon and spore density. Plants in Jupr-Mase community were suitable hosts for AMF and had higher soil carbon stock due to low disturbance and dense tree cover. Conserving remnant dry Afromontane forest have great role on harboring AMF and increased soil carbon stock which is important in increased soil carbon and climate change mitigation.